Gram Stain

Gram Stain

Preferred Specimen(s) Swabs, sputum, bronchial washing, body fluids, urine, tissue

Instructions Collect using sterile technique

Transport Container Bacterial transport tube, sterile screw cap container, or transport tube with preservative (for urine)

Transport Temperature Room termperature : 10 days for dried smear and 2 days for specimen swab  Refrigerated : Respiratory specimen and body fluids are stable upto 24 hrs & 48 hrs, respectively

Reject Criteria Received at room temperature; Frozen

Methodology Microscopic Examination

Clinical Significance The Gram Stain is a rapid, preliminary test to identify bacterial infections and to guide initial antibacterial therapy.

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