Mycobacterium, Culture

Mycobacterium, Culture

Includes Acid fast stain, Mycobacterium culture, Concentration If culture is positive, identification will be performed at an additional charge.

Preferred Specimen(s)

Sputum: Collect three separate first morning deep cough sputum specimens for three consecutive days. Submit each specimen immediately following collection.

Instructions Specimens of gastric aspirates should be neutralized with 1 gm of sodium carbonate per 15 mL of specimen before transport to the laboratory. Specimens should be delivered to the laboratory within one day of collection.

Transport Container Sterile plastic screw-capped tube

Note: Blood should be transported in sodium heparinized (green-top) tubes or tubes with SPS anticoagulant (yellow-top) tubes.

Transport Temperature

• Room temperature 48 hrs,
• Refrigerated 5 days

Reject Criteria

• Swab transport medium >5 days old
• Dry swabs
• Expired swab transport medium or port-a-cul medium
• Specimen received in formalin and/or alcohol
• Raw specimens >5 days old
• A bagged specimen that has leaked out and contaminated the exterior of the container
• 24-hour pooled sputum or urine specimens
• Urine received in Boricon tube
• Frozen samples
• Broken slides

Methodology MGIT 960

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