Vitamin D, 25-Hydroxy, CLIA

Vitamin D, 25-Hydroxy, CLIA

Includes D2, D3 and Total Vitamin D, 25-Hydroxy

Preferred Specimen(s) 1 mL serum

Instructions Collect blood by venipuncture into plain tubes (without anticoagulant). Separate the serum from the cells immediately.

Transport Container Plastic screw-cap vial

Transport Temperature Frozen; 2-8oC

Reject Criteria

• Gross hemolysis
• Gross lipemia
• Plasma

Methodology CLIA

Clinical Significance This assay should be used to monitor patients taking vitamin D supplements as well as to diagnose Vitamin D deficiency. The measurement of 25-OH Vitamin D is the best indicator of Vitamin D status in the body. This assay employs chemiluminescence and measures the total of 25-OH D2 (the analog form used to treat 25-OH D3 deficiency) and 25-OH D3( the endogenous form of the vitamin).

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